Basic Vector Information
- Vector Name:
- pPLV02
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5069 bp
- Type:
- Cloning vector
- Replication origin:
- pSa ori
- Host:
- Plants
- Source/Author:
- De Rybel B, van den Berg W, Lokerse A, Liao CY, van Mourik H, Moller B, Peris CL, Weijers D.
- Promoter:
- CaMV 35S
pPLV02 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pPLV02 vector Sequence
LOCUS 40924_35074 5069 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pPLV02, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5069) AUTHORS De Rybel B, van den Berg W, Lokerse A, Liao CY, van Mourik H, Moller B, Peris CL, Weijers D. TITLE A versatile set of ligation-independent cloning vectors for functional studies in plants JOURNAL Plant Physiol. 156 (3), 1292-1299 (2011) PUBMED 21562332 REFERENCE 2 (bases 1 to 5069) AUTHORS De Rybel B, van den Berg W, Weijers D. TITLE Direct Submission JOURNAL Submitted (04-MAY-2011) Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, Wageningen 6703HA, The Netherlands REFERENCE 3 (bases 1 to 5069) TITLE Direct Submission REFERENCE 4 (bases 1 to 5069) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant Physiol."; date: "2011"; volume: "156"; issue: "3"; pages: "1292-1299" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (04-MAY-2011) Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, Wageningen 6703HA, The Netherlands" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5069 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin complement(66..654) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(828..1640) /label=KanR /note="aminoglycoside phosphotransferase" rep_origin 1931..2366 /label=pSa ori /note="origin of replication from bacterial plasmid pSa" misc_feature 2501..2523 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA (truncated)" polyA_signal complement(2531..2707) /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" CDS complement(2812..3603) /label=NeoR/KanR /note="aminoglycoside phosphotransferase" promoter complement(3629..3974) /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" primer_bind 4176..4192 /note="M13F; M13 forward primer site" primer_bind 4196..4212 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 4219..4237 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 4263..4279 /label=KS primer /note="common sequencing primer, one of multiple similar variants" misc_feature 4300..4329 /note="LIC; ligation-independent cloning (LIC) site" terminator 4343..4591 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" promoter complement(4628..4646) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(4667..4683) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(4691..4707) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4715..4745) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4760..4781) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." misc_feature 5020..5044 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA"
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