Basic Vector Information
- Vector Name:
- pPLV01
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4716 bp
- Type:
- Cloning vector
- Replication origin:
- pSa ori
- Source/Author:
- De Rybel B, van den Berg W, Lokerse A, Liao CY, van Mourik H, Moller B, Peris CL, Weijers D.
- Promoter:
- T3
pPLV01 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pPLV01 vector Sequence
LOCUS 40924_35064 4716 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pPLV01, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4716)
AUTHORS De Rybel B, van den Berg W, Lokerse A, Liao CY, van Mourik H, Moller
B, Peris CL, Weijers D.
TITLE A versatile set of ligation-independent cloning vectors for
functional studies in plants
JOURNAL Plant Physiol. 156 (3), 1292-1299 (2011)
PUBMED 21562332
REFERENCE 2 (bases 1 to 4716)
AUTHORS De Rybel B, van den Berg W, Weijers D.
TITLE Direct Submission
JOURNAL Submitted (04-MAY-2011) Laboratory of Biochemistry, Wageningen
University, Dreijenlaan 3, Wageningen 6703HA, The Netherlands
REFERENCE 3 (bases 1 to 4716)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4716)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant
Physiol."; date: "2011"; volume: "156"; issue: "3"; pages:
"1292-1299"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(04-MAY-2011) Laboratory of Biochemistry, Wageningen University,
Dreijenlaan 3, Wageningen 6703HA, The Netherlands"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4716
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(66..654)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(828..1640)
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin 1931..2366
/label=pSa ori
/note="origin of replication from bacterial plasmid pSa"
misc_feature 2501..2523
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA
(truncated)"
terminator complement(2534..2786)
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(2823..3371)
/label=BlpR
/note="phosphinothricin acetyltransferase"
promoter complement(3413..3592)
/label=NOS promoter
/note="nopaline synthase promoter"
primer_bind 3819..3835
/note="M13F; M13 forward primer site"
primer_bind 3839..3855
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 3865..3883
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 3909..3925
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 3946..3975
/note="LIC; ligation-independent cloning (LIC) site"
terminator 3989..4237
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
promoter complement(4274..4292)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(4313..4329)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4337..4353)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4361..4392)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(4407..4428)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 4667..4691
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
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