Basic Vector Information
- Vector Name:
- pPLaR1
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5258 bp
- Type:
- Bacterial expression vector
- Replication origin:
- ori
- Source/Author:
- De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
pPLaR1 vector Map
pPLaR1 vector Sequence
LOCUS V004038 5258 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V004038
VERSION V004038
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 5258)
AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
Fonseca M, Vanhoucke M, Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5258)
AUTHORS De Schamphelaire W.
TITLE Direct Submission
JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM
REFERENCE 3 (bases 1 to 5258)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5258)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
9052, BELGIUM"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5258
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory complement(117..247)
/label="PL"
/note="PL"
/regulatory_class="promoter"
CDS 615..1427
/label="KanR"
/note="aminoglycoside phosphotransferase"
rep_origin complement(1889..2477)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(2965..3154)
/label="IS1"
/note="IS1"
CDS complement(4987..5211)
/note="Lysis protein from Escherichia phage MS2.
Accession#: P03609"
/label="Lysis protein"
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