pPL5618_pUG_MtxR vector (V004044)

LOCUS       V004044                 3760 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004044
VERSION     V004044
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   MacDonald C, Piper RC.
  TITLE     Puromycin- and methotrexate-resistance cassettes and optimized
            Cre-recombinase expression plasmids for use in yeast
  JOURNAL   Yeast 32 (5), 423-438 (2015)
   PUBMED   25688547
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   MacDonald C, Piper RC.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3760)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3760)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Sequence Builder (Lasergene) v. 8.0.3
            Sequencing Technology :: Assembled
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Yeast";
            date: "2015"; volume: "32"; issue: "5"; pages: "423-438"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-NOV-2014) Molecular Physiology "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    53..86
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        141..484
                     /label="TEF promoter"
                     /note="Ashbya gossypii TEF promoter"
     CDS             485..1045
                     /gene="DHFR"
                     /label="Dihydrofolate reductase"
                     /note="Dihydrofolate reductase from Homo sapiens.
                     Accession#: P00374"
     terminator      1054..1251
                     /label="TEF terminator"
                     /note="Ashbya gossypii TEF terminator"
     misc_feature    1311..1344
                     /label="loxP site"
                     /note="loxP site"
     protein_bind    complement(1311..1344)
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     promoter        complement(1398..1416)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1674..2262)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2436..3293)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3294..3398)
                     /label="AmpR promoter"
     promoter        3744..3760
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"