Basic Vector Information
- Vector Name:
- pPL5618_pUG_MtxR
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3760 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- MacDonald C, Piper RC.
- Promoter:
- TEF
pPL5618_pUG_MtxR vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pPL5618_pUG_MtxR vector Sequence
LOCUS V004044 3760 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V004044
VERSION V004044
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 3760)
AUTHORS MacDonald C, Piper RC.
TITLE Puromycin- and methotrexate-resistance cassettes and optimized
Cre-recombinase expression plasmids for use in yeast
JOURNAL Yeast 32 (5), 423-438 (2015)
PUBMED 25688547
REFERENCE 2 (bases 1 to 3760)
AUTHORS MacDonald C, Piper RC.
TITLE Direct Submission
REFERENCE 3 (bases 1 to 3760)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3760)
AUTHORS .
TITLE Direct Submission
COMMENT ##Assembly-Data-START##
Assembly Method :: Sequence Builder (Lasergene) v. 8.0.3
Sequencing Technology :: Assembled
##Assembly-Data-END##
SGRef: number: 1; type: "Journal Article"; journalName: "Yeast";
date: "2015"; volume: "32"; issue: "5"; pages: "423-438"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(18-NOV-2014) Molecular Physiology "
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3760
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 53..86
/label="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
promoter 141..484
/label="TEF promoter"
/note="Ashbya gossypii TEF promoter"
CDS 485..1045
/gene="DHFR"
/label="Dihydrofolate reductase"
/note="Dihydrofolate reductase from Homo sapiens.
Accession#: P00374"
terminator 1054..1251
/label="TEF terminator"
/note="Ashbya gossypii TEF terminator"
misc_feature 1311..1344
/label="loxP site"
/note="loxP site"
protein_bind complement(1311..1344)
/label="loxP"
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
promoter complement(1398..1416)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin complement(1674..2262)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2436..3293)
/label="AmpR"
/note="beta-lactamase"
promoter complement(3294..3398)
/label="AmpR promoter"
promoter 3744..3760
/label="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
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