pPKm-248 vector (V004054)

LOCUS       V004054                11558 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004054
VERSION     V004054
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11558)
  AUTHORS   Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Jian-Yu V, Chao
            SS, Hsu A, Pham V, Naghavian L, Dozier LE, Patrick G, Coleman T.
  TITLE     Biosynthesis of Orthogonal Molecules Using Ferredoxin and
            Ferredoxin-NADP+ Reductase Systems Enables Genetically Encoded PhyB
            Optogenetics
  JOURNAL   ACS Synth Biol (2018) In press
   PUBMED   29301067
REFERENCE   2  (bases 1 to 11558)
  AUTHORS   Kyriakakis P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2017) Bioengineering, UCSD, 9500 Gilman drive,
            PFBH room 251 MC0412, La Jolla, CA 92093, United States of America
REFERENCE   3  (bases 1 to 11558)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11558)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
            Biol (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-NOV-2017) Bioengineering, UCSD, 9500 Gilman drive, PFBH room 251
            MC0412, La Jolla, CA 92093, United States of America"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             1264..1338
                     /label="COX8 presequence"
                     /note="mitochondrial presequence of human cytochrome c
                     oxidase subunit VIII (Rizutto et al., 1989)"
     misc_feature    1351..2061
                     /label="tPCYA"
                     /note="tPCYA"
     misc_feature    2092..2718
                     /label="IRES"
                     /note="IRES"
     CDS             2731..2805
                     /label="COX8 presequence"
                     /note="mitochondrial presequence of human cytochrome c
                     oxidase subunit VIII (Rizutto et al., 1989)"
     CDS             3553..3609
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     misc_feature    3610..3696
                     /label="transit peptide mito"
                     /note="transit peptide mito"
     CDS             3610..3684
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label="COX8 presequence"
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      3706..3715
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3715..3756
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             3763..4056
                     /gene="petF1"
                     /label="Ferredoxin-1"
                     /note="Ferredoxin-1 from Synechococcus elongatus.
                     Accession#: P0A3D0"
     misc_feature    4057..4122
                     /label="P2A"
                     /note="P2A"
     CDS             4066..4122
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    4123..4209
                     /label="transit peptide mito(2)"
                     /note="transit peptide mito(2)"
     CDS             4123..4197
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label="COX8 presequence"
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             4228..4245
                     /label="6xHis"
                     /note="6xHis affinity tag"
     misc_feature    4252..5409
                     /label="FNR THEEB"
                     /note="FNR THEEB"
     promoter        5443..5772
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5782..6378
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             6674..6907
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7064..7288
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      7331..7340
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        7384..7488
                     /label="AmpR promoter"
     CDS             7489..8346
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8520..9108
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     LTR             9267..9900
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     promoter        10002..11179
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             11176..11217
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    11314..11431
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"