pPK121 vector (V004079)

LOCUS       V004079                 9915 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004079
VERSION     V004079
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9915)
  AUTHORS   Coy DL, Wagenbach M, Howard J.
  TITLE     Kinesin takes one 8-nm step for each ATP that it hydrolyzes
  JOURNAL   J. Biol. Chem. 274 (6), 3667-3671 (1999)
   PUBMED   9920916
REFERENCE   2  (bases 1 to 9915)
  AUTHORS   Coy DL, Wagenbach M, Howard J.
  TITLE     Mechanochemical Coupling Stoichiometry of Single Kinesin Motors
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 9915)
  AUTHORS   Coy DL, Wagenbach M, Howard J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-1998) Physiology and Biophysics, University of
            Washington, Campus Box 357290, Seattle, WA 98195-7290, USA
REFERENCE   4  (bases 1 to 9915)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 9915)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol.
            Chem."; date: "1999"; volume: "274"; issue: "6"; pages: "3667-3671"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (23-MAR-1998) Physiology and Biophysics, University of Washington,
            Campus Box 357290, Seattle, WA 98195-7290, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        494..598
                     /label="AmpR promoter"
     CDS             599..1456
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1630..2218
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2404..2546)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2651..2839)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(3614..3635)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3651..4730)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(4731..4808)
                     /label="lacI promoter"
     promoter        5117..5135
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5136..5160
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5175..5197
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5205..8129
                     /gene="Khc"
                     /label="Kinesin heavy chain"
                     /note="Kinesin heavy chain from Drosophila melanogaster.
                     Accession#: P17210"
     CDS             8154..8177
                     /codon_start=1
                     /product="co-translated peptide"
                     /label="co-translated peptide"
                     /note="modeled on Schoner, B.E. et al. (1984) PNAS USA
                     81:5403-5407"
                     /protein_id="AAD13355.1"
                     /translation="MFPLEDD"
     CDS             8178..9701
                     /gene="Klc"
                     /label="Kinesin light chain"
                     /note="Kinesin light chain from Drosophila melanogaster.
                     Accession#: P46824"
     CDS             9702..9719
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             9720..9746
                     /label="9xHis"
                     /note="9xHis affinity tag"
     CDS             9759..9776
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      9843..9890
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"