Basic Vector Information
- Vector Name:
- pPK113
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6567 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Coy DL, Wagenbach M, Howard J.
pPK113 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pPK113 vector Sequence
LOCUS V004081 6567 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V004081
VERSION V004081
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 6567)
AUTHORS Coy DL, Wagenbach M, Howard J.
TITLE Kinesin takes one 8-nm step for each ATP that it hydrolyzes
JOURNAL J. Biol. Chem. 274 (6), 3667-3671 (1999)
PUBMED 9920916
REFERENCE 2 (bases 1 to 6567)
AUTHORS Coy DL, Wagenbach M, Howard J.
TITLE Mechanochemical Coupling Stoichiometry of Single Kinesin Motors
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 6567)
AUTHORS Coy DL, Wagenbach M, Howard J.
TITLE Direct Submission
JOURNAL Submitted (13-MAR-1998) Physiology and Biophysics, University of
Washington, Campus Box 357290, Seattle, WA 98195-7290, USA
REFERENCE 4 (bases 1 to 6567)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 6567)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol.
Chem."; date: "1999"; volume: "274"; issue: "6"; pages: "3667-3671"
SGRef: number: 2; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(13-MAR-1998) Physiology and Biophysics, University of Washington,
Campus Box 357290, Seattle, WA 98195-7290, USA"
SGRef: number: 4; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6567
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 12..467
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 494..598
/label="AmpR promoter"
CDS 599..1456
/label="AmpR"
/note="beta-lactamase"
rep_origin 1630..2218
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(2404..2546)
/label="bom"
/note="basis of mobility region from pBR322"
CDS complement(2651..2839)
/label="rop"
/note="Rop protein, which maintains plasmids at low copy
number"
promoter 3348..3366
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
RBS 3398..3420
/label="RBS"
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 3428..6352
/gene="Khc"
/label="Kinesin heavy chain"
/note="Kinesin heavy chain from Drosophila melanogaster.
Accession#: P17210"
CDS 6353..6370
/label="thrombin site"
/note="thrombin recognition and cleavage site"
CDS 6371..6388
/label="6xHis"
/note="6xHis affinity tag"
CDS 6411..6428
/label="6xHis"
/note="6xHis affinity tag"
terminator 6495..6542
/label="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
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