pPK113 vector (V004081)

LOCUS       V004081                 6567 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004081
VERSION     V004081
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Coy DL, Wagenbach M, Howard J.
  TITLE     Kinesin takes one 8-nm step for each ATP that it hydrolyzes
  JOURNAL   J. Biol. Chem. 274 (6), 3667-3671 (1999)
   PUBMED   9920916
REFERENCE   2  (bases 1 to 6567)
  AUTHORS   Coy DL, Wagenbach M, Howard J.
  TITLE     Mechanochemical Coupling Stoichiometry of Single Kinesin Motors
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6567)
  AUTHORS   Coy DL, Wagenbach M, Howard J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-1998) Physiology and Biophysics, University of
            Washington, Campus Box 357290, Seattle, WA 98195-7290, USA
REFERENCE   4  (bases 1 to 6567)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6567)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol.
            Chem."; date: "1999"; volume: "274"; issue: "6"; pages: "3667-3671"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (13-MAR-1998) Physiology and Biophysics, University of Washington,
            Campus Box 357290, Seattle, WA 98195-7290, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        494..598
                     /label="AmpR promoter"
     CDS             599..1456
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1630..2218
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2404..2546)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2651..2839)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     promoter        3348..3366
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3398..3420
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3428..6352
                     /gene="Khc"
                     /label="Kinesin heavy chain"
                     /note="Kinesin heavy chain from Drosophila melanogaster.
                     Accession#: P17210"
     CDS             6353..6370
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             6371..6388
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             6411..6428
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      6495..6542
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"