Basic Vector Information
- Vector Name:
- pPIPRA589
- Antibiotic Resistance:
- Kanamycin
- Length:
- 13717 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Figueroa-Balderas RE, Chi-Ham CL, Bennett AB.
- Promoter:
- MAS
pPIPRA589 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pPIPRA589 vector Sequence
LOCUS 40924_34694 13717 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pPIPRA589, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13717) AUTHORS Figueroa-Balderas RE, Chi-Ham CL, Bennett AB. TITLE Enabling Technologies for Plant Transformation JOURNAL Unpublished REFERENCE 2 (bases 1 to 13717) AUTHORS Figueroa-Balderas RE, Chi-Ham CL, Bennett AB. TITLE Direct Submission JOURNAL Submitted (14-DEC-2011) Plant Sciences, UC Davis, One shields Avenue, Davis, CA 95616, USA REFERENCE 3 (bases 1 to 13717) TITLE Direct Submission REFERENCE 4 (bases 1 to 13717) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (14-DEC-2011) Plant Sciences, UC Davis, One shields Avenue, Davis, CA 95616, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..13717 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 26..406 /label=MAS promoter /note="mannopine synthase promoter (Velten et al., 1984)" CDS 420..1142 /codon_start=1 /gene="ipt" /product="isopentenyl phosphotransferase" /label=ipt /note="condensation of AMP and isopentenylpyrophosphate to form isopentenyl-AMP, a cytokinin" /protein_id="AFI23682.1" /translation="MDLHLIFGPTCTGKTTTAIALAQQTGLPVLSLDRVQCCPQLSTGS GRPTVEELKGTTRLYLDDRPLVEGIIAAKQAHHRLIEEVYNHEANGGLILEGGSTSLLN CMARNSYWSADFRWHIIRHKLPDQETFMKAAKARVKQMLHPAAGHSIIQELVYLWNEPR LRPILKEIDGYRYAMLFASQNQITADMLLQLDANMEGKLINGIAQEYFIHARQQEQKFP QVNAAAFDGFEGHPFGMY" gene 420..1142 /gene="ipt" /label=ipt terminator 1505..1757 /label=MAS terminator /note="mannopine synthase terminator" misc_feature 2026..2050 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" regulatory 2137..3080 /label=FMV34S /note="FMV34S" /regulatory_class="promoter" CDS 3099..3887 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" regulatory 3897..4173 /label=Mas term 3' UTR /note="Mas term 3' UTR" /regulatory_class="terminator" terminator 3908..4160 /label=MAS terminator /note="mannopine synthase terminator" regulatory 4217..5160 /label=FMV34S /note="FMV34S" /regulatory_class="promoter" CDS 5227..6507 /label=codA /note="E. coli cytosine deaminase" regulatory 6523..6826 /label=Pea E9 3' UTR /note="Pea E9 3' UTR" /regulatory_class="terminator" misc_feature 6913..6937 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 8401..9027 /label=pVS1 StaA /note="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS 9459..10529 /label=pVS1 RepA /note="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" rep_origin 10598..10792 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" misc_feature 11266..11406 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(11592..12180) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(12648..13439) /label=KanR /note="aminoglycoside phosphotransferase"
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