Basic Vector Information
- Vector Name:
- pPIPRA542
- Antibiotic Resistance:
- Kanamycin
- Length:
- 11761 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Figueroa-Balderas RE, Chi-Ham CL, Le H, Tricoli D, Sandman J, Johnson D, Reich J, Bennett AB.
- Promoter:
- MAS
pPIPRA542 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pPIPRA542 vector Sequence
LOCUS 40924_34669 11761 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pPIPRA542, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11761)
AUTHORS Figueroa-Balderas RE, Chi-Ham CL, Le H, Tricoli D, Sandman J,
Johnson D, Reich J, Bennett AB.
TITLE Constitutive expression of eIF5A3 increases high quality biomass
yield in an elite alfalfa cultivar
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 11761)
AUTHORS Figueroa-Balderas RE, Chi-Ham CL, Le H, Tricoli D, Sandman J,
Johnson D, Reich J, Bennett AB.
TITLE Direct Submission
JOURNAL Submitted (13-JAN-2015) Plant Sciences, UC Davis, One shields
Avenue, Davis, CA 95616, USA
REFERENCE 3 (bases 1 to 11761)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 11761)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(13-JAN-2015) Plant Sciences, UC Davis, One shields Avenue, Davis,
CA 95616, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..11761
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 245..269
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
promoter 362..742
/label=MAS promoter
/note="mannopine synthase promoter (Velten et al., 1984)"
CDS 762..1550
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
terminator 1571..1823
/label=MAS terminator
/note="mannopine synthase terminator"
regulatory 1874..2455
/label=histone H3
/note="histone H3"
/regulatory_class="promoter"
intron 2517..2706
/label=cat1 intron
/note="castor bean catalase intron, modified"
CDS 2701..4524
/label=GUS
/note="beta-glucuronidase"
CDS 4531..4548
/label=6xHis
/note="6xHis affinity tag"
regulatory 4579..4882
/label=pea E9
/note="pea E9"
/regulatory_class="terminator"
misc_feature 4969..4993
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 6445..7071
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS 7503..8573
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 8642..8836
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 9310..9450
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(9636..10224)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(10692..11483)
/label=KanR
/note="aminoglycoside phosphotransferase"
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