Basic Vector Information
- Vector Name:
- pPIPRA540
- Antibiotic Resistance:
- Kanamycin
- Length:
- 12117 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Figueroa-Balderas RE, Chi-Ham CL, Le H, Tricoli D, Sandman J, Johnson D, Reich J, Bennett AB.
- Promoter:
- MAS
pPIPRA540 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pPIPRA540 vector Sequence
LOCUS 40924_34664 12117 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pPIPRA540, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12117)
AUTHORS Figueroa-Balderas RE, Chi-Ham CL, Le H, Tricoli D, Sandman J,
Johnson D, Reich J, Bennett AB.
TITLE Constitutive expression of eIF5A3 increases high quality biomass
yield in an elite alfalfa cultivar
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 12117)
AUTHORS Figueroa-Balderas RE, Chi-Ham CL, Le H, Tricoli D, Sandman J,
Johnson D, Reich J, Bennett AB.
TITLE Direct Submission
JOURNAL Submitted (13-JAN-2015) Plant Sciences, UC Davis, One shields
Avenue, Davis, CA 95616, USA
REFERENCE 3 (bases 1 to 12117)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 12117)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(13-JAN-2015) Plant Sciences, UC Davis, One shields Avenue, Davis,
CA 95616, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..12117
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 245..269
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
promoter 362..742
/label=MAS promoter
/note="mannopine synthase promoter (Velten et al., 1984)"
CDS 762..1550
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
terminator 1571..1823
/label=MAS terminator
/note="mannopine synthase terminator"
regulatory 1869..2812
/label=FMV34S
/note="FMV34S"
/regulatory_class="promoter"
intron 2884..3073
/label=cat1 intron
/note="castor bean catalase intron, modified"
CDS 3068..4891
/label=GUS
/note="beta-glucuronidase"
CDS 4898..4915
/label=6xHis
/note="6xHis affinity tag"
regulatory 4946..5246
/label=pea E9
/note="pea E9"
/regulatory_class="terminator"
misc_feature 5325..5349
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
misc_feature 5462..5569
/label=T-DNA right border
/note="T-DNA right border"
CDS 6801..7427
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS 7859..8929
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 8998..9192
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 9666..9806
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(9992..10580)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(11048..11839)
/label=KanR
/note="aminoglycoside phosphotransferase"
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