pPIGA3 vector (V004099)

LOCUS       40924_34619        9375 bp DNA     circular SYN 18-DEC-2018
DEFINITION  PiggyBac donor vector pPIGA3 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Mitsutake H, Kobayashi J.
  TITLE     piggyBac-mediated stable transformation of cultured Bombyx mori 
            cells using in vitro synthesized transposase mRNA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9375)
  AUTHORS   Mitsutake H, Kobayashi J.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2010) Contact:Jun Kobayashi Yamaguchi University, 
            Faculty of Agriculture; Yoshida 1677-1, Yamaguchi, Yamaguchi 
            753-8515, Japan URL 
            :http://www.agr.yamaguchi-u.ac.jp/~member/kobayashi/pe.html
REFERENCE   3  (bases 1 to 9375)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9375)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-OCT-2010) Contact:Jun Kobayashi Yamaguchi University, Faculty of
            Agriculture"; volume: " Yoshida 1677-1, Yamaguchi, Yamaguchi 
            753-8515, Japan URL :http"; pages: 
            "//www.agr.yamaguchi-u.ac.jp/~member/kobayashi/pe.htm"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2375..2410
                     /label=piggyBac 5' inverted terminal repeat
                     /note="piggyBac 5' inverted terminal repeat"
     promoter        3131..3583
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             3682..4278
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     misc_feature    4335..4640
                     /gene="PGK"
                     /label=PGK polyadenylation sequence
                     /note="PGK polyadenylation sequence"
     gene            4335..4640
                     /gene="PGK"
                     /label=PGK
     gene            4658..5313
                     /gene="A3"
                     /label=A3
     regulatory      4658..4784
                     /gene="A3"
                     /label=actin A3
                     /note="actin A3"
                     /regulatory_class="promoter"
     exon            4785..4853
                     /gene="A3"
                     /note="actin A3"
     intron          4854..5313
                     /gene="A3"
                     /note="actin A3"
     CDS             5343..6059
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    6185..6306
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(6313..6768)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6795..6899
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6942..7239
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     misc_feature    8105..8128
                     /note="piggyBac transposase polyadenylation sequence; 
                     transposase"
     repeat_region   8230..8292
                     /label=piggyBac 3' inverted terminal repeat
                     /note="piggyBac 3' inverted terminal repeat"
     primer_bind     complement(8981..8997)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"