pPICBT3 vector (V004103)

MSDS

Basic Vector Information

Vector Name:: pPICBT3

Antibiotic Resistance:: Ampicillin

Length:: 4678 bp

Type:: Transformation vector

Replication origin:: ori

Source/Author:: Oertel W, Weissgerber A.

pPICBT3 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pPICBT3 vector Sequence

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Download GeneBank File(.gb)

LOCUS       40924_34565        4678 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector pPICBT3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Transformation of Ulva mutabilis Foyn with plasmids integrating into
            the chromosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2007) Biochemistry, Genetics and Microbiology, 
            Division of Genetics, University of Regensburg, Universitaetsstr. 
            31, Regensburg, Bavaria 93053, Germany
REFERENCE   3  (bases 1 to 4678)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4678)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-OCT-2007) Biochemistry, Genetics and Microbiology, Division of 
            Genetics, University of Regensburg, Universitaetsstr. 31, 
            Regensburg, Bavaria 93053, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..546
                     /label=Ulva RbcS promoter
                     /note="Ulva RbcS promoter"
                     /regulatory_class="promoter"
     intron          555..717
                     /gene="ble"
                     /number=1
                     /note="derived from Ulva RbcS"
     CDS             complement(865..1521)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(1522..1624)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     intron          1718..2050
                     /gene="ble"
                     /number=1
                     /note="derived from Ulva RbcS"
     CDS             2049..2414
                     /label=BleoR
                     /note="antibiotic-binding protein"
     regulatory      2418..2806
                     /label=Ulva RbcS terminator
                     /note="Ulva RbcS terminator"
                     /regulatory_class="terminator"
     promoter        2894..2998
                     /label=AmpR promoter
     CDS             2999..3856
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4030..4618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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