pPICBT2 vector (V004104)

LOCUS       40924_34560        5510 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector pPICBT2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Transformation of Ulva mutabilis Foyn with plasmids integrating into
            the chromosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2007) Biochemistry, Genetics and Microbiology, 
            Division of Genetics, University of Regensburg, Universitaetsstr. 
            31, Regensburg, Bavaria 93053, Germany
REFERENCE   3  (bases 1 to 5510)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5510)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-OCT-2007) Biochemistry, Genetics and Microbiology, Division of 
            Genetics, University of Regensburg, Universitaetsstr. 31, 
            Regensburg, Bavaria 93053, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..546
                     /label=Ulva mutabilis RbcS promoter
                     /note="Ulva mutabilis RbcS promoter"
                     /regulatory_class="promoter"
     CDS             complement(865..1521)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(1522..1624)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2049..2414
                     /label=BleoR
                     /note="antibiotic-binding protein"
     regulatory      2418..2806
                     /label=Ulva mutabilis RbcS terminator
                     /note="Ulva mutabilis RbcS terminator"
                     /regulatory_class="terminator"
     primer_bind     3375..3391
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(3395..3451)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3461..3477)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3485..3501)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3509..3539)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3554..3575)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3863..4451)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4625..5482)
                     /label=AmpR
                     /note="beta-lactamase"