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PpcleAmiR4-7 vector (V004168#)

Basic Vector Information

Basic Vector Information
Vector Name PpcleAmiR4-7 Antibiotic Resistance Ampicillin
Length 8026 bp Type UNVERIFIED: Expression vector
Source Whitewoods CD, Cammarata J, Nemec Venza Z, Sang S, Crook AD, Aoyama T, Wang XY, Waller M, Kamisugi Y, Cuming AC, Szovenyi P, Nimchuk ZL, Roeder AH, Scanlon MJ, Harrison CJ.

PpcleAmiR4-7 vector Vector Map

M13 fwd f1 ori AmpR promoter AmpR ori CAP binding site lac promoter lac operator M13 rev T7 promoter CaMV 35S promoter HygR CaMV poly(A) signal attB2 OCS terminator SP6 promoter

PpcleAmiR4-7 vector Sequence

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Download GeneBank File(.gb)

LOCUS       Exported                8026 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  UNVERIFIED: Expression vector PpcleAmiR4-7, complete sequence.
ACCESSION   MH310735
VERSION     .
KEYWORDS    UNVERIFIED.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Whitewoods CD, Cammarata J, Nemec Venza Z, Sang S, Crook AD, Aoyama 
            T, Wang XY, Waller M, Kamisugi Y, Cuming AC, Szovenyi P, Nimchuk ZL,
            Roeder AH, Scanlon MJ, Harrison CJ.
  TITLE     CLAVATA Was a Genetic Novelty for the Morphological Innovation of 3D
            Growth in Land Plants
  JOURNAL   Curr. Biol. 28 (15), 2365-2376 (2018)
  PUBMED    30033333
REFERENCE   2  (bases 1 to 8026)
  AUTHORS   Whitewoods CD, Cammarata J, Nemec Venza Z, Sang S, Crook AD, Aoyama 
            T, Waller M, Kamisugi Y, Cuming AC, Szovenyi P, Nimchuk ZL, Roeder 
            AHK., Scanlon MJ, Harrison CJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2018) Biological Sciences, University of Bristol, 
            24 Tyndall Avenue, Bristol BS81TQ, UK
REFERENCE   3  (bases 1 to 8026)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     GenBank staff is unable to verify sequence and/or annotation 
            provided by the submitter.
            ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Expression vector PpcleAmiR4-7"
                     /mol_type="other DNA"
                     /db_xref="taxon:2293868"
     primer_bind     complement(17..33)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(213..641)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        668..772
                     /gene="bla"
                     /label=AmpR promoter
     CDS             773..1633
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1804..2392
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2680..2701
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2716..2746
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2754..2770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2778..2794
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2825..2843
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2926..3271
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3288..4313
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    4523..4699
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    complement(4762..4786)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      7257..7963
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(7996..8014)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"

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