pPBAsyn vector (V004172)

LOCUS       V004172                10456 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004172
VERSION     V004172
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Ghosh IN, Landick R.
  TITLE     OptSSeq: High-throughput sequencing readout of growth enrichment
            defines optimal gene expression elements for homoethanologenesis
  JOURNAL   ACS Synth Biol (2016) In press
   PUBMED   27404024
REFERENCE   2  (bases 1 to 10456)
  AUTHORS   Ghosh IN, Landick RC.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2016) DOE Great Lakes Bioenergy Research Center,
            University of Wisconsin-Madison, 1552 University Avenue, Madison, WI
            53726, United States
REFERENCE   3  (bases 1 to 10456)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10456)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
            Biol (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-JUL-2016) DOE Great Lakes Bioenergy Research Center, University
            of Wisconsin-Madison, 1552 University Avenue, Madison, WI 53726,
            United States"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      35..96
                     /function="Stationary phase promoter"
                     /label="Dps100"
                     /note="Dps100"
                     /regulatory_class="promoter"
     regulatory      108..181
                     /function="Expressed in anaerobic conditions"
                     /label="YdfZ promoter"
                     /note="YdfZ promoter"
                     /regulatory_class="promoter"
     CDS             221..1924
                     /gene="pdc"
                     /label="Pyruvate decarboxylase"
                     /note="Pyruvate decarboxylase from Zymomonas mobilis subsp.
                     mobilis (strain ATCC 31821 / ZM4 / CP4). Accession#:
                     P06672"
     CDS             1987..3135
                     /gene="adhB"
                     /label="Alcohol dehydrogenase 2"
                     /note="Alcohol dehydrogenase 2 from Zymomonas mobilis
                     subsp. mobilis (strain ATCC 31821 / ZM4 / CP4). Accession#:
                     P0DJA2"
     CDS             3167..4177
                     /gene="adhA"
                     /label="Alcohol dehydrogenase 1"
                     /note="Alcohol dehydrogenase 1 from Zymomonas mobilis
                     subsp. mobilis (strain ATCC 31821 / ZM4 / CP4). Accession#:
                     P20368"
     CDS             4211..4993
                     /gene="pfyP"
                     /label="Blue-light photoreceptor"
                     /note="Blue-light photoreceptor from Bacillus subtilis
                     (strain 168). Accession#: O34627"
     protein_bind    complement(5001..5034)
                     /label="FRT (minimal)"
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     CDS             5153..5965
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     protein_bind    complement(6062..6095)
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     regulatory      6108..6159
                     /label="P22 terminator"
                     /note="P22 terminator"
                     /regulatory_class="terminator"
     promoter        6232..6260
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             6449..6979
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     rep_origin      8265..9034
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             9035..9694
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     primer_bind     10404..10420
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        10430..10448
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"