pFSPGW vector (V006182)

LOCUS       40924_20571       13566 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway vector pFSPGW, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13566)
  AUTHORS   Zhang C, Galbraith DW.
  TITLE     RNA interference-mediated gene knockdown within specific cell types
  JOURNAL   Plant Mol. Biol. 80 (2), 169-176 (2012)
  PUBMED    22740284
REFERENCE   2  (bases 1 to 13566)
  AUTHORS   Zhang C, Galbraith DW.
  TITLE     Gateway-adapted T-DNA binary vectors derived from pFGC5941 
            (AY310901)
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 13566)
  AUTHORS   Zhang C, Galbraith DW.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2005) Department of Plant Sciences, University of 
            Arizona, 303 Forbes Building, Tucson, AZ 85721-0036, USA
REFERENCE   4  (bases 1 to 13566)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 13566)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2012"; volume: "80"; issue: "2"; pages: "169-176"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (05-OCT-2005) Department of Plant Sciences, University of Arizona, 
            303 Forbes Building, Tucson, AZ 85721-0036, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(376..924)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    1323..1393
                     /note="synthetic linker including 6 unique restriction
                     sites"
     misc_feature    1415..1469
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    1500..1619
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1649..1679
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1733..2389
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2734..3036
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3080..3204)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          3215..4563
                     /label=chsA intron
                     /note="chalcone synthase A intron from Petunia hybrida"
     protein_bind    4610..4734
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4778..5080)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(5425..6081)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(6135..6165)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(6195..6314)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      6338..7045
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(7070..7086)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7289..7313
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8613..9239
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             9676..10740
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      10809..11003
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    11347..11487
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(11673..12261)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12351..13142)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"