pFR-Bgal vector (V006197)

LOCUS       40924_20486        7601 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFR-Bgal, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Vaillancourt P, Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Road, La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 7601)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7601)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (13-OCT-1998) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Road, La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    33..127
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     regulatory      144..149
                     /regulatory_class="TATA_box"
     CDS             309..3353
                     /label=lacZ
                     /note="beta-galactosidase"
     intron          3972..4037
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4167..4187
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4612..4746
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5059..5647)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5821..6678)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6679..6783)
                     /label=AmpR promoter
     rep_origin      6810..7265
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_feature    7377..7577
                     /label=pA#1
                     /note="pA#1"
     polyA_signal    7455..7576
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"