pFNK-804-lacO-lacI vector (V006206)

LOCUS       V006206                 5821 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006206
VERSION     V006206
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Karig D, Martini KM, Lu T, DeLateur NA, Goldenfeld N, Weiss R.
  TITLE     Stochastic Turing patterns in a synthetic bacterial population
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2018) In press
   PUBMED   29891706
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Karig DK, Lu T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2018) Research and Development Department, Johns
            Hopkins University Applied Physics Laboratory, 11100 Johns Hopkins
            Rd, Laurel, MD 20723, USA
REFERENCE   3  (bases 1 to 5821)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5821)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-MAY-2018) Research and Development Department, Johns Hopkins
            University Applied Physics Laboratory, 11100 Johns Hopkins Rd,
            Laurel, MD 20723, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(47..769)
                     /gene="rhlR"
                     /label="HTH-type quorum-sensing regulator RhlR"
                     /note="HTH-type quorum-sensing regulator RhlR from
                     Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP
                     104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
                     Accession#: P54292"
     RBS             776..787
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     regulatory      complement(779..787)
                     /label="RBSII"
                     /note="RBSII"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(802..844)
                     /regulatory_class="promoter"
     regulatory      complement(812..817)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(834..840)
                     /regulatory_class="minus_35_signal"
     protein_bind    921..937
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      946..962
                     /label="RBSH"
                     /note="RBSH"
                     /regulatory_class="ribosome_binding_site"
     CDS             963..1673
                     /label="lambda repressor"
                     /note="phage lambda repressor"
     CDS             1680..1712
                     /label="ssrA tag (LVA)"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     RBS             1720..1731
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     regulatory      1720..1728
                     /label="RBSII"
                     /note="RBSII"
                     /regulatory_class="ribosome_binding_site"
     CDS             1738..2451
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     terminator      2713..2799
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(2889..3968)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(3969..4046)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     rep_origin      complement(4143..4688)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(4802..4896)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(4930..5721)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"