pFN28K HaloTag(R) CMV-neo vector (V006212)

LOCUS       40924_20366        5535 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Flexi vector pFN28K HaloTag(R) CMV-neo, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   McNamara B, Kennedy J.
  TITLE     pFN28K HaloTag(R) CMV-neo Flexi Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   McNamara B, Kennedy J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUN-2011) Scientific Communications, Promega 
            Corporation, 5500 E. Cheryl Parkway, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 5535)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5535)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-JUN-2011) Scientific Communications, Promega Corporation, 5500 
            E. Cheryl Parkway, Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1067..1957
                     /label=HaloTag(R)
                     /note="modified bacterial dehalogenase that forms covalent
                     bonds with chloroalkane derivatives"
     CDS             1970..1990
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    1997..2004
                     /note="SgfI site; used in Flexi vector cloning"
     CDS             2028..2360
                     /label=barnase
                     /note="ribonuclease from Bacillus amyloliquefaciens"
     misc_feature    2365..2372
                     /note="PmeI site; used in Flexi vector cloning"
     misc_feature    2373..2428
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     polyA_signal    complement(2533..2654)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2899..3256
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        3271..3318
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             3350..4141
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4208..4256
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4495..5083)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5199..5482
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"