pFGUS3 vector (V006259)

LOCUS       V006259                11545 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006259
VERSION     V006259
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11545)
  AUTHORS   Zhang C, Chen ZJ.
  TITLE     Regulation of NAC2 expression by miR164c mediates fertilizer
            application-induced delay of leaf senescence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11545)
  AUTHORS   Zhang C, Chen ZJ.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11545)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11545)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. Suite 8
            Coverage              :: complete
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-APR-2013) Section of Molecular Cell "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label="MAS terminator"
                     /note="mannopine synthase terminator"
     CDS             complement(376..924)
                     /label="BlpR"
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(930..1310)
                     /label="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     terminator      1325..1577
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1579..1681
                     /note="Multiple Cloning Sites (MCS); NOS terminator
                     sequence to insulate MAS promoter from the promoter to be
                     cloned"
     CDS             1818..2252
                     /note="Histone H2B.10 from Arabidopsis thaliana.
                     Accession#: Q9FFC0"
                     /label="Histone H2B.10"
     intron          2286..2475
                     /label="cat1 intron"
                     /note="castor bean catalase intron, modified"
     CDS             2470..4293
                     /label="GUS"
                     /note="beta-glucuronidase"
     terminator      4313..5020
                     /label="OCS terminator"
                     /note="octopine synthase terminator"
     primer_bind     complement(5049..5065)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    5268..5292
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6592..7218
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             7655..8719
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      8788..8982
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9326..9466
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9652..10240)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10330..11121)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"