pFastBac 1-Gag1-M1-GFLGL-rMVP vector (V006298)

LOCUS       V006298                 7733 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006298
VERSION     V006298
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Ding K, Zhang X, Mrazek J, Kickhoefer VA, Lai M, Ng HL, Yang OO,
            Rome LH, Zhou ZH.
  TITLE     Solution Structures of Engineered Vault Particles
  JOURNAL   Structure (2018) In press
   PUBMED   29551289
REFERENCE   2  (bases 1 to 7733)
  AUTHORS   Ding K, Zhang X, Mrazek J, Kickhoefer VA, Lai M, Ng HL, Yang OO,
            Rome LH, Zhou H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2018) Bioengineering, University of California,
            Los Angeles, 405 Hilgard Ave, Los Angeles, CA 90034, USA
REFERENCE   3  (bases 1 to 7733)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7733)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Structure
            (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-MAR-2018) Bioengineering, University of California, Los Angeles,
            405 Hilgard Ave, Los Angeles, CA 90034, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        484..588
                     /label="AmpR promoter"
     CDS             589..1446
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1620..2208
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     mobile_element  complement(2511..2735)
                     /label="Tn7R"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2805..3335)
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     promoter        complement(3524..3552)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4287..6869
                     /gene="Mvp"
                     /label="Major vault protein"
                     /note="Major vault protein from Rattus norvegicus.
                     Accession#: Q62667"
     polyA_signal    7223..7357
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(7386..7551)
                     /label="Tn7L"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"