pEZY202 vector (V006396)

LOCUS       40924_19001       12414 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pEZY202, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12414)
  AUTHORS   Guo F, Wang Y, Zhang YZ.
  TITLE     Construction of two recombination yeast two-hybrid vectors by in 
            vitro recombination
  JOURNAL   Mol. Biotechnol. 36 (1), 38-43 (2007)
  PUBMED    17827536
REFERENCE   2  (bases 1 to 12414)
  AUTHORS   Guo F, Wang Y, Zhang Y-Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2007) Biological, Chemical, and Physical Sciences,
            Illinois Institute of Technology, 3101 South Dearborn St., Chicago, 
            IL 60616, USA
REFERENCE   3  (bases 1 to 12414)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12414)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Biotechnol."; date: "2007"; volume: "36"; issue: "1"; pages: "38-43"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-SEP-2007) Biological, Chemical, and Physical Sciences, Illinois 
            Institute of Technology, 3101 South Dearborn St., Chicago, IL 60616,
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        807..1511
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1538..2143
                     /codon_start=1
                     /label=LexA
                     /note="E. coli DNA-binding protein that represses genes
                     involved in the SOS response to DNA damage"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     protein_bind    2156..2280
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2712..3014)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(3362..4018)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(4019..4121)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(4299..4423)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4457..4644
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     rep_origin      5376..6718
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(8365..9021)
                     /codon_start=1
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(9022..9209)
                     /label=HIS3 promoter
     promoter        10274..10378
                     /label=AmpR promoter
     CDS             10379..11236
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11410..11998
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(12184..12324)
                     /label=bom
                     /note="basis of mobility region from pBR322"