pEXPR49 vector (V006414)

LOCUS       V006414                 7758 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006414
VERSION     V006414
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Siciliano V.
  TITLE     Engineering modular intracellular protein sensor-actuator devices
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   Siciliano V.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2018) Biological Engineering, Massachusetts
            Institute of Technology, 500 Tech Square, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 7758)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7758)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-MAR-2018) Biological Engineering, Massachusetts Institute of
            Technology, 500 Tech Square, Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(222..810)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(984..1841)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1842..1946)
                     /label="AmpR promoter"
     rep_origin      complement(1972..2427)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2569..2585
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2595..2613
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2628..3431
                     /label="HA-L"
                     /note="left homology arm from the adeno-associated virus
                     integration site (AAVS1) within intron 1 of the human
                     PPP1R12C gene"
     misc_feature    3510..3535
                     /label="SA"
                     /note="splice acceptor site"
     protein_bind    4207..4227
                     /label="attB4"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     promoter        4252..5424
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     protein_bind    5446..5470
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5484..5741
                     /gene="tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate HXB2). Accession#: P04608"
     protein_bind    complement(5791..5815)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5970..6025
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(6386..6402)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    6410..6426
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6434..6464)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6479..6500
                     /label="CAP binding site"
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    6673..7509
                     /label="HA-R"
                     /note="right homology arm from the adeno-associated virus
                     integration site (AAVS1) within intron 1 of the human
                     PPP1R12C gene"
     promoter        complement(7539..7557)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7578..7594)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7602..7618)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7626..7656)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7671..7692)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."