pEX2kanMX4 vector (V006433)

LOCUS       V006433                10609 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006433
VERSION     V006433
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10609)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10609)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10609)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10609)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..840
                     /label="ARS1"
                     /note="S. cerevisiae autonomously replicating sequence
                     ARS1/ARS416"
     rep_origin      94..838
                     /label="ARS1"
                     /note="ARS1"
     terminator      complement(842..1089)
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     CDS             2103..2552
                     /gene="ANB1"
                     /label="Eukaryotic translation initiation factor 5A-2"
                     /note="Eukaryotic translation initiation factor 5A-2 from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P19211"
     promoter        3627..3847
                     /label="URA3 promoter"
     CDS             3848..4648
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      5577..6919
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     misc_feature    7061..7201
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7387..7975)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8149..9006)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9007..9111)
                     /label="AmpR promoter"
     gene            complement(9145..10501)
                     /label="kanMX"
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"