pES4 vector (V006527)

LOCUS       40924_17596        5209 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pES4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Zhang X, Wang XY, Jia YL, Guo X, Wang YF, Wang TY.
  TITLE     A Vector Based on the Chicken Hypersensitive Site 4 Insulator 
            Element Replicates Episomally in Mammalian Cell
  JOURNAL   Curr Gene Ther (2017) In press
  PUBMED    28155604
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Wang T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2017) Department of Biochemistry and Molecular 
            Biology, Xinxiang Medical University, Jinsui Road, Xinxiang, Henan 
            453000, Xinxiang
REFERENCE   3  (bases 1 to 5209)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5209)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Gene 
            Ther (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2017) Department of Biochemistry and Molecular Biology, 
            Xinxiang Medical University, Jinsui Road, Xinxiang, Henan 453000, 
            Xinxiang"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1329
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1808..1873
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    1997..2118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2125..2580)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2607..2711
                     /label=AmpR promoter
     promoter        2713..3070
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3105..3896
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4131..4178
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4507..5095
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"