pHC65 vector (V005590)

LOCUS       40924_24248        9177 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHC65, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   Chou H-H., Berthet J, Marx CJ.
  TITLE     Growth rate modulates the selective advantage of recurrent 
            transposition mutations arising from experimental populations grown 
            in a metal-limited environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9177)
  AUTHORS   Chou H-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2008) Organismic and Evolutionary Biology, Harvard
            University, 16 Divinity Avenue, Biolab 3079, Cambridge, MA 02138, 
            USA
REFERENCE   3  (bases 1 to 9177)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9177)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2008) Organismic and Evolutionary Biology, Harvard 
            University, 16 Divinity Avenue, Biolab 3079, Cambridge, MA 02138, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..861)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(862..966)
                     /label=AmpR promoter
     misc_feature    1005..1628
                     /note="5' upstream region of Methylobacterium extorquens
                     AM1 icuA"
     misc_feature    1634..2182
                     /note="3' downstream region of Methylobacterium extorquens
                     AM1 icuA"
     CDS             complement(2792..3160)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(3193..3302)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        3939..3967
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             4015..5202
                     /label=TcR
                     /note="tetracycline efflux protein"
     CDS             complement(5386..6042)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             complement(6143..7561)
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     promoter        complement(7562..8007)
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     rep_origin      complement(8419..9007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"