pGT2 vector (V005868)

LOCUS       40924_22779        3267 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGT2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Park HK, Zeng C.
  TITLE     Construction of an XcmI-generated T vector bearing green fluorescent
            protein marker for direct cloning of PCR products
  JOURNAL   Anal. Biochem. 360 (1), 144-145 (2007)
  PUBMED    17113027
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Park HK, Zeng C.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2006) Bio. Sci., Univ. of Wisconsin-Milwaukee, 
            3209 N. Maryland Ave., Milwaukee, WI 53211, USA
REFERENCE   3  (bases 1 to 3267)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3267)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal. 
            Biochem."; date: "2007"; volume: "360"; issue: "1"; pages: "144-145"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-SEP-2006) Bio. Sci., Univ. of Wisconsin-Milwaukee, 3209 N. 
            Maryland Ave., Milwaukee, WI 53211, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    52..73
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        88..118
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    126..142
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     150..166
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    214..228
                     /gene="gfpuv4-2aa"
                     /label=XcmI restriction site #1
                     /note="XcmI restriction site #1"
     CDS             234..947
                     /codon_start=1
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALLKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    948..962
                     /label=XcmI restriction site #2
                     /note="XcmI restriction site #2"
     promoter        1076..1180
                     /label=AmpR promoter
     CDS             1181..2038
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2212..2800
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2986..3126)
                     /label=bom
                     /note="basis of mobility region from pBR322"