pGloSensor-20F cAMP vector (V005922)

LOCUS       40924_22408        6990 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pGloSensor-20F cAMP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Stecha PF, McNamara B, Binkowski BF.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAY-2008) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd, Madison, WI 53711-5399, USA
REFERENCE   2  (bases 1 to 6990)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6990)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (29-MAY-2008) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Rd, Madison, WI 53711-5399, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(8..865)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    970..1018
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    1032..1123
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     enhancer        1275..1653
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1654..1857
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        1890..1908
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1922..4027
                     /codon_start=1
                     /product="GloSensor cAMP"
                     /label=GloSensor cAMP
                     /protein_id="ACF75745.1"
                     /translation="MPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYV
                     NNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQH
                     PNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVD
                     EVPKGLTGKLDARKIREILIKAKKGSSGGSGGSGGGMYESFIESLPFLKSLEFSERLKV
                     VDVIGTKVYNDGEQIIAQGDSADSFFIVESGEVKITMKRKGKSEVEENGAVEIARCSRG
                     QYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVAL
                     FGTNMDIVAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYA
                     EYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERE
                     LLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHL
                     PPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQI
                     IPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPT
                     LFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAI
                     LITPEGV"
     polyA_signal    complement(4069..4190)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4384..4741
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4772..5806
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     misc_feature    5833..5881
                     /note="synthetic poly(A) signal/transcriptional pause site"
     polyA_signal    5833..5881
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     primer_bind     5948..5967
                     /label=RVprimer4
                     /note="RVprimer4"
     rep_origin      complement(6208..6795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"