pGloSensor-10F[TEV] vector (V005923)

LOCUS       40924_22403        4989 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGloSensor-10F[TEV], complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Wigdal SS, Frackman S, Slater M.
  TITLE     pGloSensor-10F[TEV] Control Plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Wigdal SS, Frackman S, Slater M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2008) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 4989)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4989)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAY-2008) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Rd., Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        23..41
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      46..63
                     /label=T7 RNA polymerase promoter
                     /note="T7 RNA polymerase promoter"
                     /regulatory_class="promoter"
     promoter        46..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           65..201
                     /label=BYDV 5' UTR
                     /note="barley yellow dwarf virus 5' untranslated region; 
                     required for function of the cap-independent 3' translation
                     enhancer (Guo et al., 2001)"
     CDS             1153..1173
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    1896..2002
                     /label=BYDV 3' TE
                     /note="cap-independent 3' translation enhancer from barley 
                     yellow dwarf virus (Wang et al., 1997)"
     terminator      2107..2154
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2383..2487
                     /label=AmpR promoter
     CDS             2488..3345
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(3506..4094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4210..4493
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
     terminator      4723..4809
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4901..4928
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"