pCMV Flag WT-PTEN vector (V006650)

MSDS

Basic Vector Information

- - Vector Name:
  - pCMV Flag WT-PTEN
- - Antibiotic Resistance:
  - Kanamycin
- - Length:
  - 5539 bp
- - Type:
  - Mammalian Expression
- - Replication origin:
  - ori
- - Selection Marker:
  - Neomycin (select with G418)
- - Copy Number:
  - High Copy
- - Promoter:
  - CMV
- - Cloning Method:
  - Restriction Enzyme
- - 5' Primer:
  - T3
- - 3' Primer:
  - T7

pCMV Flag WT-PTEN vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pCMV Flag WT-PTEN vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pCMV_Flag_WT-PTE        5539 bp DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV Flag WT-PTEN.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Mosessian S, Avliyakulov NK, Mulholland DJ, Boontheung P, Loo JA, Wu
            H
  TITLE     Analysis of PTEN complex assembly and identification of hnRNP C as a
            component of the PTEN-associated complex.
  JOURNAL   J Biol Chem. 2009 Sep 9. ():.
  PUBMED    19740742
REFERENCE   2  (bases 1 to 5539)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5539)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2009 Sep 9. ():."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        621..639
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      674..683
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             683..706
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             722..1930
                     /gene="PTEN"
                     /label=PTEN
                     /note="Phosphatidylinositol 3,4,5-trisphosphate
                     3-phosphatase and dual-specificity protein phosphatase PTEN
                     from Canis lupus familiaris. Accession#: P60483"
     primer_bind     complement(1969..1985)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2042..2060)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2334..2455
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2462..2917)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2944..3046
                     /label=AmpR promoter
     promoter        3050..3407
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3442..4233
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(4424..4443)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4468..4515
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4844..5432
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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