PGK1p-Csy4-pA vector (V005975)

Basic Vector Information

      • Vector Name:
      • PGK1p-Csy4-pA
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 4199 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK.

PGK1p-Csy4-pA vector Vector Map

PGK1p-Csy4-pA4199 bp60012001800240030003600f1 oriPGK promoter6xHisTEV siteCsy4oriampicillin resistance protein

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

PGK1p-Csy4-pA vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                4199 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector PGK1p-Csy4-pA, complete sequence.
ACCESSION   KJ796485
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK.
  TITLE     Multiplexed and programmable regulation of gene networks with an 
            integrated RNA and CRISPR/Cas toolkit in human cells
  JOURNAL   Mol. Cell 54 (4), 698-710 (2014)
  PUBMED    24837679
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Perli SD.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2014) Electrical Engineering and Computer Science,
            Massachusetts Institute of Technology, 77, Massachusetts Avenue, 
            Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 4199)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Cloning vector PGK1p-Csy4-pA"
                     /mol_type="other DNA"
                     /db_xref="taxon:1520552"
     rep_origin      72..527
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        660..1159
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             1201..1218
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1219..1239
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     gene            1252..1809
                     /gene="Csy4"
                     /label=Csy4
     regulatory      1976..2197
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SV40"
     gene            1976..2197
                     /gene="SV40"
                     /label=SV40
     polyA_signal    1985..2106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2520..3108)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3279..4139)
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="AIJ03084.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4140..45)
                     /gene="bla"
                     /label=AmpR promoter

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