pGFtef3-hph vector (V006027)

LOCUS       40924_21633        5240 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pGFtef3-hph DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Sato M, Kurahashi A, Nishibori K, Fujimori F.
  TITLE     Development of a transformation system for the edible mushroom 
            Grifola frondosa: demonstrating heterologous gene expression and 
            RNAi-mediated gene silencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Sato M, Kurahashi A, Nishibori K, Fujimori F.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2014) Contact:Masayuki Sato Yukiguni Maitake Co. 
            Ltd., Research and Development; 89 Yokawa, Minamiuonuma, Niigata 
            949-6695, Japan URL :http://www.maitake.co.jp
REFERENCE   3  (bases 1 to 5240)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5240)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-FEB-2014) Contact:Masayuki Sato Yukiguni Maitake Co. Ltd., 
            Research and Development; 89 Yokawa, Minamiuonuma, Niigata 949-6695,
            Japan URL :http://www.maitake.co.jp"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..727
                     /gene="Gf.TEF3"
                     /regulatory_class="promoter"
     gene            1..727
                     /gene="Gf.TEF3"
                     /label=Gf.TEF3
     CDS             728..1750
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     regulatory      1754..2550
                     /gene="Gf.TEF3"
                     /regulatory_class="terminator"
     gene            1754..2550
                     /gene="Gf.TEF3"
                     /label=Gf.TEF3
     primer_bind     complement(2598..2614)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2622..2638)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2646..2676)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2691..2712)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3000..3588)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3762..4619)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4620..4724)
                     /label=AmpR promoter
     primer_bind     5198..5214
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"