pGEX6p-2rbs vector (V006048)

LOCUS       40924_21509        5056 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGEX6p-2rbs, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   De Antoni A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-OCT-2014) IFOM, Via Adamello 16, Milan 20131, Italy
REFERENCE   2  (bases 1 to 5056)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5056)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (01-OCT-2014) IFOM, Via Adamello 16, Milan 20131, Italy"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    219..235
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      244..253
                     /regulatory_class="ribosome_binding_site"
     CDS             258..911
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             918..941
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     RBS             986..1008
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        1359..1463
                     /label=AmpR promoter
     CDS             1464..2321
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2495..3083
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3327..3404
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3405..4484
                     /label=lacI
                     /note="lac repressor"
     protein_bind    4500..4521
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4536..4566
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4574..4590
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4610..4783
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"