pGDR11-Tgo vector (V006068)

LOCUS       V006068                 7078 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006068
VERSION     V006068
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Dunn MR, Otto CE, Fenton KE, Chaput JC.
  TITLE     Transference as a Mechanism for Expanding and Improving Polymerase
            Function
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7078)
  AUTHORS   Dunn MR, Otto CE, Fenton KE, Chaput JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2015) The Biodesign Institute, Arizona State
            University, 727 E Tyler Street, Tempe, AZ 85287, USA
REFERENCE   3  (bases 1 to 7078)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7078)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-JAN-2015) The Biodesign Institute, Arizona State University, 727
            E Tyler Street, Tempe, AZ 85287, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..2319
                     /gene="pol"
                     /label="DNA polymerase"
                     /note="DNA polymerase from Thermococcus gorgonarius.
                     Accession#: P56689"
     terminator      2358..2452
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             2496..3152
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     terminator      3220..3306
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    complement(3367..3388)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3404..4483)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(4482..4559)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    4751..4891
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5077..5665)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5839..6696)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6697..6801)
                     /label="AmpR promoter"
     promoter        6911..6955
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     operon          6961..6981
                     /operon="LacO"
                     /label="LacO operon"
     protein_bind    6963..6979
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6998..7009
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             7028..7045
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             7061..7078
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"