pG2RNAi2 vector (V006135)

LOCUS       40924_20840       10824 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pG2RNAi2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10824)
  AUTHORS   Noon JB, Maier TR, Baum TJ.
  TITLE     HgGLAND18 is a malaria-like effector of the soybean cyst nematode 
            that is important for virulence on soybean and efficiently targets 
            host cell nucleoli for suppression of innate immunity
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10824)
  AUTHORS   Noon JB, Maier TR, Baum TJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2015) Plant Pathology and Microbiology, Iowa State
            University, 351 Bessey Hall, Ames, IA 50011, USA
REFERENCE   3  (bases 1 to 10824)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10824)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-OCT-2015) Plant Pathology and Microbiology, Iowa State 
            University, 351 Bessey Hall, Ames, IA 50011, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(114..138)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     334..350
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      432..1348
                     /label=Glycine max polyubiquitin (PGmUBI)
                     /note="Glycine max polyubiquitin (PGmUBI)"
                     /regulatory_class="promoter"
     misc_feature    1359..1366
                     /label=AscI restriction site
                     /note="AscI restriction site"
     misc_feature    1367..1374
                     /label=SwaI restriction site
                     /note="SwaI restriction site"
     misc_feature    1378..2306
                     /label=GUS linker
                     /note="GUS linker"
     misc_feature    2306..2311
                     /label=BamHI restriction site
                     /note="BamHI restriction site"
     misc_feature    2314..2318
                     /label=AvrII restriction site
                     /note="AvrII restriction site"
     regulatory      2319..2573
                     /label=rbcS
                     /note="rbcS"
                     /regulatory_class="terminator"
     regulatory      2917..3434
                     /label=CsVMV
                     /note="CsVMV"
                     /regulatory_class="promoter"
     CDS             3440..4156
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      4178..4430
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4472..4488)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4496..4512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4520..4550)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4565..4586)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    complement(4763..4787)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5080..5892
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      5990..6578
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(6764..6904)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7248..7442)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7511..8575)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9012..9638)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"