pFUSE-LIGHT vector (V006151)

LOCUS       V006151                 5983 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006151
VERSION     V006151
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Loppnau PA, Graslund S, Arrowsmith CH.
  TITLE     A pipeline to generate recombinant antibodies to human target
            proteins involved in epigenetic mechanisms
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   Loppnau PA, Graslund S, Arrowsmith CH.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2012) Structural Genomics Consortium, University
            of Toronto, Mars Centre, South Tower, 101 College St, Room 700,
            Toronto, Ontario M5G 1L7, Canada
REFERENCE   3  (bases 1 to 5983)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5983)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-NOV-2012) Structural Genomics Consortium, University of Toronto,
            Mars Centre, South Tower, 101 College St, Room 700, Toronto, Ontario
            M5G 1L7, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..242
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     LTR             255..523
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     556..615
                     /label="IL-2 signal sequence"
                     /note="human interleukin-2 signal sequence"
     misc_feature    616..753
                     /note="partial coding sequence human Immunoglobulin light
                     chain, kappa type"
     misc_feature    694..708
                     /label="5'-In-Fusion cloning site"
                     /note="5'-In-Fusion cloning site"
     protein_bind    complement(765..798)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        813..1258
                     /label="sacB promoter"
                     /note="sacB promoter and control region"
     CDS             1259..2677
                     /label="SacB"
                     /note="secreted levansucrase that renders bacterial growth
                     sensitive to sucrose"
     misc_feature    2737..2757
                     /note="partial coding sequence human Immunoglobulin light
                     chain, kappa type"
     misc_feature    2742..2757
                     /label="3'-In-Fusion cloning site"
                     /note="3'-In-Fusion cloning site"
     CDS             2761..3078
                     /label="hIg-kappa-CL"
                     /note="Human immunoglobulin kappa light chain constant
                     region"
     polyA_signal    complement(3118..3239)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(3343..3737)
                     /label="beta-globin poly(A)"
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(3749..4168)
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     regulatory      complement(4218..4260)
                     /label="EM2KC bacterial promoter"
                     /note="EM2KC bacterial promoter"
                     /regulatory_class="promoter"
     regulatory      complement(4378..4640)
                     /label="human ferritin light chain mammalian cell promoter"
                     /note="human ferritin light chain mammalian cell promoter"
                     /regulatory_class="promoter"
     enhancer        complement(4748..5051)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(5131..5719)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     polyA_signal    5827..5875
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    5886..5977
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"