SARS-CoV-2 S HexaPro vector (V007232)

Basic Vector Information

      • Vector Name:
      • SARS-CoV-2 S HexaPro
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 8376 bp
      • Type:
      • Mammalian Expression
      • Copy Number:
      • High Copy

SARS-CoV-2 S HexaPro vector Vector Map

SARS-CoV-2 S HexaPro8376 bp400800120016002000240028003200360040004400480052005600600064006800720076008000CMV enhancerchimeric intronHRV 3C site8xHisStrep-Tag IIStrep-Tag IIBglob-pA-Rbeta-globin poly(A) signalM13 revlac operatorlac promoterCAP binding siteL4440oriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

SARS-CoV-2 S HexaPro vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                8376 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Mammalian expression vector for expression of the SARS-CoV-2 spike 
            HexaPro variant.
ACCESSION   .
VERSION     .
KEYWORDS    SARS-CoV-2 S HexaPro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Hsieh CL, Goldsmith JA, Schaub JM, DiVenere AM, Kuo HC, Javanmardi 
            K, Le KC, Wrapp D, Lee AG, Liu Y, Chou CW, Byrne PO, Hjorth CK, 
            Johnson NV, Ludes-Meyers J, Nguyen AW, Park J, Wang N, Amengor D, 
            Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS
  TITLE     Structure-based design of prefusion-stabilized SARS-CoV-2 spikes.
  JOURNAL   Science. 2020 Sep 18;369(6510):1501-1505. doi: 
            10.1126/science.abd0826. Epub 2020 Jul 23.
  PUBMED    32703906
REFERENCE   2  (bases 1 to 8376)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abd0826"; journalName: "Science"; date: 
            "2020-09-18- 18"; volume: "369"; issue: "6510"; pages: "1501-1505"
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             5486..5509
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5513..5536
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             5543..5566
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5606..5629
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(5731..5750)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5796..5851
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5850..5869)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6212..6228)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6212..6228)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6225..6247)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6236..6252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6260..6290)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6305..6326
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6455..6472)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6626..7214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6706..6725)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7385..8245)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8008..8027
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8246..8350)
                     /gene="bla"
                     /label=AmpR promoter

This page is informational only.