Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V007273 | ISceI-GR-RFP | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
ISceI-GR-RFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Soutoglou E, Dorn JF, Sengupta K, Jasin M, Nussenzweig A, Ried T, Danuser G, Misteli T. Positional stability of single double-strand breaks in mammalian cells. Nat Cell Biol. 2007 Jun;9(6):675-82. doi: 10.1038/ncb1591. Epub 2007 May 7.
ISceI-GR-RFP vector Sequence
LOCUS 40924_1419 6276 bp DNA circular SYN 13-JAN-2022 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6276) TITLE Direct Submission REFERENCE 2 (bases 1 to 6276) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..6276 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 68..371 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 372..575 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" CDS 620..1294 /label=DsRed-Monomer /note="monomeric derivative of DsRed fluorescent protein (Strongin et al., 2007)" CDS 1319..2023 /gene="SCEI" /label=SCEI /note="Intron-encoded endonuclease I-SceI from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). Accession#: P03882" polyA_signal 3071..3192 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(3199..3654) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3681..3785 /label=AmpR promoter promoter 3787..4144 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 4179..4970 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" polyA_signal 5205..5252 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5581..6169 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"