ISceI-GR-RFP vector (V007273)

Price Information

Cat No. Plasmid Name Availability Add to cart
V007273 ISceI-GR-RFP In stock (lyophilized plasmid)

Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

      • Vector Name:
      • ISceI-GR-RFP
      • Antibiotic Resistance:
      • Kanamycin
      • Length:
      • 6276 bp
      • Type:
      • Mammalian Expression Vectors
      • Selection Marker:
      • DsRed-Monomer
      • Promoter:
      • CMV
      • Growth Strain(s):
      • DH5alpha
      • Growth Temperature:
      • 37℃

ISceI-GR-RFP vector Vector Map

ISceI-GR-RFP6276 bp30060090012001500180021002400270030003300360039004200450048005100540057006000CMV enhancerCMV promoterDsRed-MonomerSCEISV40 poly(A) signalf1 oriAmpR promoterSV40 promoterNeoR/KanRHSV TK poly(A) signalori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

  • Soutoglou E, Dorn JF, Sengupta K, Jasin M, Nussenzweig A, Ried T, Danuser G, Misteli T. Positional stability of single double-strand breaks in mammalian cells. Nat Cell Biol. 2007 Jun;9(6):675-82. doi: 10.1038/ncb1591. Epub 2007 May 7.

ISceI-GR-RFP vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_1419        6276 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6276)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             620..1294
                     /label=DsRed-Monomer
                     /note="monomeric derivative of DsRed fluorescent protein 
                     (Strongin et al., 2007)"
     CDS             1319..2023
                     /gene="SCEI"
                     /label=SCEI
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c). 
                     Accession#: P03882"
     polyA_signal    3071..3192
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3199..3654)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3681..3785
                     /label=AmpR promoter
     promoter        3787..4144
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4179..4970
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5205..5252
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5581..6169
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"