FLAG.PKCepsilon vector (V007296)

MSDS

Basic Vector Information

- - Vector Name:
  - FLAG.PKCepsilon
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 7616 bp
- - Type:
  - Mammalian Expression
- - Replication origin:
  - ori
- - Selection Marker:
  - Neomycin (select with G418)
- - Copy Number:
  - High Copy
- - Promoter:
  - SV40
- - Cloning Method:
  - Restriction Enzyme
- - 5' Primer:
  - T7

FLAG.PKCepsilon vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

FLAG.PKCepsilon vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       FLAG.PKCepsilon.        7616 bp DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    FLAG.PKCepsilon.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Cenni V, Doppler H, Sonnenburg ED, Maraldi N, Newton AC, Toker A
  TITLE     Regulation of novel protein kinase C epsilon by phosphorylation.
  JOURNAL   Biochem J. 2002 May 1. 363(Pt 3):537-45.
  PUBMED    11964154
REFERENCE   2  (bases 1 to 7616)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7616)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2002 May 1. 363(Pt 3):537-45."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(42..61)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     enhancer        233..612
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        613..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        861..879
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             917..940
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             941..3148
                     /gene="PRKCE"
                     /label=PRKCE
                     /note="Protein kinase C epsilon type from Homo sapiens. 
                     Accession#: Q02156"
     promoter        complement(3167..3185)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3211..3435
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3481..3909
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3923..4252
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4319..5110
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5287..5420
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5457..5473)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5481..5497)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5505..5535)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5550..5571)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(5688..5705)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5859..6447)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6621..7478)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7479..7583)
                     /label=AmpR promoter

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