Basic Vector Information
- Vector Name:
- pLV-mitoDsRed
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7824 bp
- Type:
- Mammalian Expression, Lentiviral
- Replication origin:
- ori
- Copy Number:
- High Copy
- Promoter:
- RSV
- Cloning Method:
- Restriction Enzyme
- 5' Primer:
- CMV-F
- 3' Primer:
- WPRE-R
pLV-mitoDsRed vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLV-mitoDsRed vector Sequence
LOCUS V007321 7824 bp DNA circular SYN 13-MAY-2021
DEFINITION Exported.
ACCESSION V007321
VERSION V007321
KEYWORDS pLV-mitoDsRed.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 7824)
AUTHORS Kitay BM, McCormack R, Wang Y, Tsoulfas P, Zhai RG
TITLE Mislocalization of neuronal mitochondria reveals regulation of
Wallerian degeneration and NMNAT/WLDS-mediated axon protection
independent of axonal mitochondria.
JOURNAL Hum Mol Genet. 2013 Jan 24.
PUBMED 23314018
REFERENCE 2 (bases 1 to 7824)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 7824)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol
Genet. 2013 Jan 24."
SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7824
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 21..125
/label="AmpR promoter"
CDS 126..983
/label="AmpR"
/note="beta-lactamase"
rep_origin 1157..1745
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1899..1916
/label="L4440"
/note="L4440 vector, forward primer"
protein_bind 2033..2054
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2069..2099
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 2107..2123
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2131..2147
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 2168..2186
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 2214..2440
/label="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
LTR 2441..2621
/label="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 2668..2793
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 3286..3519
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 3704..3748
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 3897..3938
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
misc_feature 4015..4131
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 4154..4457
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 4458..4661
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 4658..4682
/label="LNCX"
/note="Human CMV promoter, forward primer"
CDS 5075..5752
/label="DsRed1"
/note="wild-type DsRed"
misc_feature 5881..6469
/label="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 6556..6789
/label="3' LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 6861..6995
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 7022..7157
/label="SV40 ori"
/note="SV40 origin of replication"
promoter complement(7178..7196)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(7206..7222)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 7364..7819
/direction=RIGHT
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
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