1136- Desmoplakin-GFP vector (V007420)

MSDS

Basic Vector Information

- - Vector Name:
  - 1136- Desmoplakin-GFP
- - Antibiotic Resistance:
  - Kanamycin
- - Length:
  - 13661 bp
- - Type:
  - Mammalian Expression
- - Replication origin:
  - ori
- - Copy Number:
  - High Copy
- - Promoter:
  - SV40
- - Cloning Method:
  - Restriction Enzyme
- - 5' Primer:
  - CMV-Fwd
- - 3' Primer:
  - EGFP-N

1136- Desmoplakin-GFP vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

1136- Desmoplakin-GFP vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       1136-_Desmoplaki       13661 bp DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    1136- Desmoplakin-GFP.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13661)
  AUTHORS   Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, 
            Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew 
            TL, Green KJ
  TITLE     Desmoplakin assembly dynamics in four dimensions: multiple phases 
            differentially regulated by intermediate filaments and actin.
  JOURNAL   J Cell Biol. 2005 Dec 19;171(6):1045-59.
  PUBMED    16365169
REFERENCE   2  (bases 1 to 13661)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 13661)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell
            Biol."; date: "2005-12-19"; volume: "171(6)"; pages: "1045-59"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             983..9595
                     /gene="DSP"
                     /label=DSP
                     /note="Desmoplakin from Homo sapiens. Accession#: P15924"
     CDS             9614..10330
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    10456..10577
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(10584..11039)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        11066..11170
                     /label=AmpR promoter
     promoter        11172..11529
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             11564..12355
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(12546..12565)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    12590..12637
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      12966..13554
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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