Basic Vector Information
- Vector Name:
- pMSCV-hygro-STING
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8118 bp
- Type:
- Mammalian Expression, Retroviral
- Replication origin:
- ori
- Copy Number:
- High Copy
- Promoter:
- MSCV
- Cloning Method:
- Restriction Enzyme
- 5' Primer:
- MSCV
pMSCV-hygro-STING vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMSCV-hygro-STING vector Sequence
LOCUS pMSCV-hygro-STIN 8118 bp DNA circular SYN 13-MAY-2021
DEFINITION Express STING for IFN-beta luciferase reporter assay..
ACCESSION .
VERSION .
KEYWORDS pMSCV-hygro-STING.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8118)
AUTHORS Cerboni S, Jeremiah N, Gentili M, Gehrmann U, Conrad C, Stolzenberg
MC, Picard C, Neven B, Fischer A, Amigorena S, Rieux-Laucat F, Manel
N
TITLE Intrinsic antiproliferative activity of the innate sensor STING in T
lymphocytes.
JOURNAL J Exp Med. 2017 Jun 5;214(6):1769-1785. doi: 10.1084/jem.20161674.
Epub 2017 May 8.
PUBMED 28484079
REFERENCE 2 (bases 1 to 8118)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 8118)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1084/jem.20161674"; journalName: "J Exp Med"; date: "2017-06-5-
5"; volume: "214"; issue: "6"; pages: "1769-1785"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8118
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..515
/label=MSCV
/note="Murine embryonic stem cell virus promoter including
the enhancer and promoter region of PCMV virus and 5'
untranslated region of dl-587rev retrovirus"
misc_feature 579..920
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 987..1403
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
CDS 1416..2552
/gene="STING1"
/label=STING1
/note="Stimulator of interferon genes protein from Homo
sapiens. Accession#: Q86WV6"
promoter 2578..3077
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
CDS 3095..4117
/label=HygR
/note="aminoglycoside phosphotransferase from E. coli"
LTR 4478..4992
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind complement(5187..5203)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5211..5241)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(5256..5277)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5394..5411)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5565..6153)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6327..7184)
/label=AmpR
/note="beta-lactamase"
promoter complement(7185..7289)
/label=AmpR promoter
primer_bind 7357..7375
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(7413..7435)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 7535..7554
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7748..7770
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 7898..7917
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
LTR 8117..8118
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
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