1432 pcDNA3 flag MAGI1c vector (V006610)

Basic Vector Information

      • Vector Name:
      • 1432 pcDNA3 flag MAGI1c
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 9835 bp
      • Type:
      • Mammalian Expression
      • Selection Marker:
      • Neomycin (select with G418)
      • Copy Number:
      • High Copy
      • Cloning Method:
      • Restriction Enzyme
      • 5' Primer:
      • T7

1432 pcDNA3 flag MAGI1c vector Vector Map

1432 pcDNA3 flag MAGI1c9835 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400880092009600CMV enhancerCMV promoterT7FLAGFLAGSP6 promoterBGH-revf1 oripBABE 3'NeoR/KanRSV40 poly(A) signalM13 revlac operatorlac promoterCAP binding siteL4440oriAmpRAmpR promoterpRS-marker

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

1432 pcDNA3 flag MAGI1c vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                9835 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    1432 pcDNA3 flag MAGI1c
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Vazquez F, Grossman SR, Takahashi Y, Rokas MV, Nakamura N, Sellers 
            WR
  TITLE     Phosphorylation of the PTEN tail acts as an inhibitory switch by 
            preventing its recruitment into a protein complex.
  JOURNAL   J Biol Chem. 2001 Dec 28. 276(52):48627-30.
  PUBMED    11707428
REFERENCE   2  (bases 1 to 9835)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 Dec 28. 276(52):48627-30."
FEATURES             Location/Qualifiers
     source          1..9835
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        173..552
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        553..756
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     707..727
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     801..820
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        801..819
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             845..868
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             872..895
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(5326..5344)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5327..5344)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5364..5381)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5370..5594
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5640..6068
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5727..5746)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5937..5958
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6077..6097)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6082..6411
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6262..6397
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6324..6343
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6478..7272
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6532..6551)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7142..7161
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7446..7567
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7483..7502)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7537..7556
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7616..7632)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7616..7632)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7629..7651)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7640..7656
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7664..7694)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7709..7730
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7847..7864)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8018..8606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8098..8117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8777..9637)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9400..9419
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9638..9742)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(9817..9835,1))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"

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