Basic Vector Information
- Vector Name:
- MP6
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 6681 bp
- Type:
- Bacterial Expression
- Replication origin:
- CloDF13 ori
- Copy Number:
- High Copy
- Promoter:
- araBAD
- Cloning Method:
- Ligation Independent Cloning
- 5' Primer:
- pBAD-F
- 3' Primer:
- CAT-F
MP6 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
MP6 vector Sequence
LOCUS MP6. 6681 bp DNA circular SYN 13-MAY-2021
DEFINITION Untargetted Bacterial Mutagenesis.
ACCESSION .
VERSION .
KEYWORDS MP6.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6681)
AUTHORS Badran AH, Liu DR
TITLE Development of potent in vivo mutagenesis plasmids with broad
mutational spectra.
JOURNAL Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
PUBMED 26443021
REFERENCE 2 (bases 1 to 6681)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 6681)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat
Commun."; date: "2015-10-7"; pages: "
10.1038/ncomms9425"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6681
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..285
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
CDS 335..1063
/gene="dnaQ"
/label=dnaQ
/note="DNA polymerase III subunit epsilon from Escherichia
coli (strain K12). Accession#: P03007"
CDS 1095..1928
/gene="dam"
/label=dam
/note="DNA adenine methylase from Escherichia coli (strain
K12). Accession#: P0AEE8"
CDS 1960..2502
/gene="seqA"
/label=seqA
/note="Negative modulator of initiation of replication from
Escherichia coli (strain K12). Accession#: P0AFY8"
CDS 2534..3061
/gene="mprA"
/label=mprA
/note="Transcriptional repressor MprA from Escherichia coli
(strain K12). Accession#: P0ACR9"
CDS 3093..3344
/label=UGI
/note="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
CDS 3377..4000
/label=PmCDA1
/note="cytidine deaminase from the sea lamprey Petromyzon
marinus (Rogozin et al., 2007)"
terminator complement(4030..4061)
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
CDS complement(4080..4736)
/label=CmR
/note="chloramphenicol acetyltransferase"
rep_origin complement(4889..5627)
/direction=LEFT
/label=CloDF13 ori
/note="Plasmids containing the CloDF13 (CDF) origin of
replication can be propagated in E. coli cells that contain
additional plasmids with compatible origins."
terminator complement(5720..5763)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(5780..6655)
/label=araC
/note="L-arabinose regulatory protein"
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