7TFP CDH1 reporter vector (V006706)

Price Information

Cat No. Plasmid Name Availability Add to cart
V006706 7TFP CDH1 reporter In stock, 1 week for quality controls

Buy one, get one free!

Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.

Basic Vector Information

Vector Name:
7TFP CDH1 reporter
Antibiotic Resistance:
Ampicillin
Length:
9174 bp
Type:
Mammalian Expression, Lentiviral, Luciferase
Replication origin:
ori
Selection Marker:
Puromycin
Copy Number:
High Copy
Promoter:
RSV
Cloning Method:
Restriction Enzyme
3' Primer:
LucNRev

7TFP CDH1 reporter vector Vector Map

7TFP CDH1 reporter9174 bp40080012001600200024002800320036004000440048005200560060006400680072007600800084008800minPluciferaseSV40 promoterPuroRWPRE3' LTR (Delta-U3)SV40 poly(A) signalSV40 oriT7 promoterM13 fwdf1 oriAmpR promoterAmpRoriL4440CAP binding sitelac promoterlac operatorM13 revT3 promoterRSV promoter5' LTR (truncated)HIV-1 PsiRREgp41 peptideProtein TatcPPT/CTS

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

7TFP CDH1 reporter vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V006706                 9174 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V006706
VERSION     V006706
KEYWORDS    7TFP CDH1 reporter.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9174)
  AUTHORS   Pattabiraman DR, Bierie B, Kober KI, Thiru P, Krall JA, Zill C,
            Reinhardt F, Tam WL, Weinberg RA
  TITLE     Activation of PKA leads to mesenchymal-to-epithelial transition and
            loss of tumor-initiating ability.
  JOURNAL   Science. 2016 Mar 4;351(6277):aad3680. doi: 10.1126/science.aad3680.
   PUBMED   26941323
REFERENCE   2  (bases 1 to 9174)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9174)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science.";
            date: "2016-03-4"; pages: "
            10.1126/science.aad3680"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        325..356
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             418..2067
                     /label="luciferase"
                     /note="firefly luciferase"
     promoter        2094..2423
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2433..3029
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    3099..3687
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             3774..4007
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4079..4213
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      4240..4375
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(4396..4414)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4424..4440)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      4582..5037
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5063..5167
                     /label="AmpR promoter"
     CDS             5168..6025
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6199..6787
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     6941..6958
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     protein_bind    7075..7096
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7111..7141
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7149..7165
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7173..7189
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7210..7228
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7256..7482
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7483..7663
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7710..7835
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    8328..8561
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             8746..8790
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             8939..8980
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    9057..9174
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"