MSCV-(pBabe mcs)- human p210BCR-ABL-IRES-GFP vector (V006838)

MSDS

Basic Vector Information

- - Vector Name:
  - MSCV-(pBabe mcs)- human p210BCR-ABL-IRES-GFP
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 13502 bp
- - Type:
  - Mammalian Expression, Retroviral
- - Replication origin:
  - ori
- - Copy Number:
  - High Copy
- - Promoter:
  - MSCV
- - Cloning Method:
  - Restriction Enzyme
- - 5' Primer:
  - pLXSN5'
- - 3' Primer:
  - pCDH-rev

MSCV-(pBabe mcs)- human p210BCR-ABL-IRES-GFP vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

MSCV-(pBabe mcs)- human p210BCR-ABL-IRES-GFP vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       MSCV-(pBabe_mcs)       13502 bp DNA     circular SYN 13-MAY-2021
DEFINITION  retroviral mammalian expression vector for human p210BCR-ABL and 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    MSCV-(pBabe mcs)- human p210BCR-ABL-IRES-GFP.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13502)
  TITLE     Roussel lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13502)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 13502)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     CDS             4319..7630
                     /gene="ABL1"
                     /label=ABL1
                     /note="Tyrosine-protein kinase ABL1 from Homo sapiens. 
                     Accession#: P00519"
     misc_feature    8452..9013
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             9016..9732
                     /label=EGFP
                     /note="enhanced GFP"
     LTR             9897..10411
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem
                     cell virus"
     protein_bind    10573..10589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10597..10627)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10642..10663)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(10780..10797)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10951..11539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11713..12570)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(12571..12675)
                     /label=AmpR promoter
     primer_bind     12743..12761
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     primer_bind     complement(12799..12821)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12921..12940
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     13134..13156
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13284..13303
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI,
                     reverse primer"

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