CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1 vector (V006907)

Price Information

Cat No. Plasmid Name Availability Add to cart
V006907 CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1 In stock, 1 week for quality controls

Buy one, get one free!

Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.

Basic Vector Information

Vector Name:
CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1
Antibiotic Resistance:
Ampicillin
Length:
13286 bp
Type:
Lentiviral
Replication origin:
ori
Selection Marker:
zsGreen1
Promoter:
EF-1α
Cloning Method:
Restriction Enzyme
5' Primer:
NA
3' Primer:
NA

CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1 vector Vector Map

CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen113286 bp6001200180024003000360042004800540060006600720078008400900096001020010800114001200012600132003' LTRHIV-1 PsiRREgp41 peptideProtein TatcPPT/CTSEF-1-alpha promoterKozak sequenceSV40 NLSSV40 NLSIRES2WPRE3' LTRM13 revlac operatorlac promoterCAP binding siteL4440oriAmpRAmpR promoterSV40 poly(A) signal

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V006907                13286 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V006907
VERSION     V006907
KEYWORDS    CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13286)
  AUTHORS   Liu X, Chen Y, Zhang Y, Liu Y, Liu N, Botten GA, Cao H, Orkin SH,
            Zhang MQ, Xu J
  TITLE     Multiplexed capture of spatial configuration and temporal dynamics
            of locus-specific 3D chromatin by biotinylated dCas9.
  JOURNAL   Genome Biol. 2020 Mar 5;21(1):59. doi: 10.1186/s13059-020-01973-w.
   PUBMED   32138752
REFERENCE   2  (bases 1 to 13286)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 13286)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome
            Biol."; date: "2020-03-5"; pages: "
            10.1186/s13059-020-01973-w"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2338..3519
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     regulatory      3558..3567
                     /label="Kozak sequence"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3573..3593
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             3591..7694
                     /label="dCas9"
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
     CDS             7704..7724
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label="SV40 NLS"
                     /translation="PKKKRKV"
     misc_feature    7972..8551
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             8546..9238
                     /label="ZsGreen1"
                     /note="Zoanthus green fluorescent protein"
     misc_feature    9255..9843
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             10050..10683
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10811..10827)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(10811..10827)
                     /label="M13 Reverse"
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10824..10846)
                     /label="M13/pUC Reverse"
                     /note="In lacZ gene"
     protein_bind    10835..10851
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10859..10889)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10904..10925)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(11042..11059)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11213..11801)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(11975..12832)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(12833..12937)
                     /label="AmpR promoter"
     polyA_signal    12985..13119
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"