E1b-GFP-Tol2 vector (V006993)

Basic Vector Information

      • Vector Name:
      • E1b-GFP-Tol2
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 7602 bp
      • Type:
      • Zebrafish enhancer assay vector
      • Replication origin:
      • ori
      • Copy Number:
      • High Copy
      • Promoter:
      • E1b
      • Cloning Method:
      • Restriction Enzyme
      • 5' Primer:
      • 3' Primer:

E1b-GFP-Tol2 vector Vector Map

E1b-GFP-Tol27602 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600690072007500AmpR promoterf1 oriM13 fwdT7 promoterbeta-globin intronEGFPSV40 poly(A) signalSK primerT3 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpR

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

E1b-GFP-Tol2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_710        7602 bp DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Birnbaum RY, Clowney EJ, Agamy O, Kim MJ, Zhao J, Yamanaka T, 
            Pappalardo Z, Clarke SL, Wenger AM, Nguyen L, Gurrieri F, Everman 
            DB, Schwartz CE, Birk OS, Bejerano G, Lomvardas S, Ahituv N
  TITLE     Coding exons function as tissue-specific enhancers of nearby genes.
  JOURNAL   Genome Res. 2012 Jun;22(6):1059-68. Epub 2012 Mar 22.
  PUBMED    22442009
REFERENCE   2  (bases 1 to 7602)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7602)
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome
            Res."; date: "2012-06"; volume: "22(6)"; pages: "1059-68. Epub 2012
            Mar 22"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(287..391)
                     /label=AmpR promoter
     rep_origin      complement(417..872)
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1014..1030
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
     promoter        1040..1058
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          3555..4127
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             4207..4923
                     /note="enhanced GFP"
     polyA_signal    complement(4971..5105)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5775..5791)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
     promoter        complement(5828..5846)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5867..5883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
     protein_bind    complement(5891..5907)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5915..5945)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5960..5981)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6269..6857)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
     CDS             complement(join(7031..7602,1..286))

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