DP6 vector (V007025)

Basic Vector Information

      • Vector Name:
      • DP6
      • Antibiotic Resistance:
      • Chloramphenicol
      • Length:
      • 8639 bp
      • Type:
      • Bacterial Expression
      • Copy Number:
      • High Copy

DP6 vector Vector Map

DP68639 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400araCpBAD ReverserrnB T1 terminatorrrnB T2 terminatortet operatorpIIICloDF13 oriCmRtonB terminatorPmCDA1UGImprAseqAdamdnaQaraBAD promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

DP6 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       DP6.        8639 bp DNA     circular SYN 13-MAY-2021
DEFINITION  Untargetted bacterial drift.
ACCESSION   .
VERSION     .
KEYWORDS    DP6.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8639)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 8639)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8639)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Commun."; date: "2015-10-7"; pages: "
            10.1038/ncomms9425"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..876
                     /label=araC
                     /note="L-arabinose regulatory protein"
     primer_bind     complement(929..946)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse
                     primer"
     terminator      1099..1185
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1277..1304
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     protein_bind    1468..1486
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1556..2827
                     /label=M13 gene III
                     /note="pIII"
     rep_origin      2987..3725
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             3878..4534
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      complement(4553..4584)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     CDS             complement(4614..5237)
                     /label=PmCDA1
                     /note="cytidine deaminase from the sea lamprey Petromyzon 
                     marinus (Rogozin et al., 2007)"
     CDS             complement(5270..5521)
                     /label=UGI
                     /note="uracil-DNA glycosylase inhibitor from a Bacillus
                     subtilis bacteriophage (Mol et al., 1995)"
     CDS             complement(5553..6080)
                     /gene="mprA"
                     /label=mprA
                     /note="Transcriptional repressor MprA from Escherichia coli
                     (strain K12). Accession#: P0ACR9"
     CDS             complement(6112..6654)
                     /gene="seqA"
                     /label=seqA
                     /note="Negative modulator of initiation of replication from
                     Escherichia coli (strain K12). Accession#: P0AFY8"
     CDS             complement(6686..7519)
                     /gene="dam"
                     /label=dam
                     /note="DNA adenine methylase from Escherichia coli (strain
                     K12). Accession#: P0AEE8"
     CDS             complement(7551..8279)
                     /gene="dnaQ"
                     /label=dnaQ
                     /note="DNA polymerase III subunit epsilon from Escherichia
                     coli (strain K12). Accession#: P03007"
     promoter        complement(8329..8613)
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"

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