Basic Vector Information
DP6 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
DP6 vector Sequence
LOCUS DP6. 8639 bp DNA circular SYN 13-MAY-2021 DEFINITION Untargetted bacterial drift. ACCESSION . VERSION . KEYWORDS DP6. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8639) AUTHORS Badran AH, Liu DR TITLE Development of potent in vivo mutagenesis plasmids with broad mutational spectra. JOURNAL Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425. PUBMED 26443021 REFERENCE 2 (bases 1 to 8639) TITLE Direct Submission REFERENCE 3 (bases 1 to 8639) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun."; date: "2015-10-7"; pages: " 10.1038/ncomms9425" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..8639 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 1..876 /label=araC /note="L-arabinose regulatory protein" primer_bind complement(929..946) /label=pBAD Reverse /note="For vectors with E. coli araBAD promoter, reverse primer" terminator 1099..1185 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 1277..1304 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" protein_bind 1468..1486 /label=tet operator /note="bacterial operator O2 for the tetR and tetA genes" CDS 1556..2827 /label=M13 gene III /note="pIII" rep_origin 2987..3725 /label=CloDF13 ori /note="Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins." CDS 3878..4534 /label=CmR /note="chloramphenicol acetyltransferase" terminator complement(4553..4584) /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" CDS complement(4614..5237) /label=PmCDA1 /note="cytidine deaminase from the sea lamprey Petromyzon marinus (Rogozin et al., 2007)" CDS complement(5270..5521) /label=UGI /note="uracil-DNA glycosylase inhibitor from a Bacillus subtilis bacteriophage (Mol et al., 1995)" CDS complement(5553..6080) /gene="mprA" /label=mprA /note="Transcriptional repressor MprA from Escherichia coli (strain K12). Accession#: P0ACR9" CDS complement(6112..6654) /gene="seqA" /label=seqA /note="Negative modulator of initiation of replication from Escherichia coli (strain K12). Accession#: P0AFY8" CDS complement(6686..7519) /gene="dam" /label=dam /note="DNA adenine methylase from Escherichia coli (strain K12). Accession#: P0AEE8" CDS complement(7551..8279) /gene="dnaQ" /label=dnaQ /note="DNA polymerase III subunit epsilon from Escherichia coli (strain K12). Accession#: P03007" promoter complement(8329..8613) /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)"
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