Basic Vector Information
- Vector Name:
- pMSCV-F-del Casp9.IRES.GFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7813 bp
- Type:
- Retroviral vectors
- Replication origin:
- ori
- Selection Marker:
- EGFP
- Promoter:
- MSCV
pMSCV-F-del Casp9.IRES.GFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMSCV-F-del Casp9.IRES.GFP vector Sequence
LOCUS 40924_32320 7813 bp DNA circular SYN 13-JAN-2022
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7813)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 7813)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7813
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..516
/label=MSCV
/note="Murine embryonic stem cell virus promoter including
the enhancer and promoter region of PCMV virus and 5'
untranslated region of dl-587rev retrovirus"
misc_feature 580..921
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 988..1404
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
CDS 1428..1748
/label=DmrB
/note="F36V mutant of FK506-binding protein FKBP12"
CDS 2625..2651
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/note="HA"
/translation="YPYDVPDYA"
misc_feature 2686..3246
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 3249..3965
/label=EGFP
/note="enhanced GFP"
LTR 4130..4645
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind complement(4807..4823)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4831..4861)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(4876..4897)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(5185..5773)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5947..6804)
/label=AmpR
/note="beta-lactamase"
promoter complement(6805..6909)
/label=AmpR promoter
primer_bind 7383..7399
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
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